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Flow cytometric analysis of the apoptotic of H9c2 cells with Annexin V-FITC indicated that pre-treatment with tert-butylhydroquinone (tBHQ) significantly prevented ethanol (EtOH)-induced apoptosis. Apoptosis was assessed in H9c2 cells cultured in control medium (control), exposed to 200 mM EtOH or 5 µM tBHQ (tBHQ) alone, or exposed to EtOH and pre-treated with tBHQ (EtOH + tBHQ). The values are expressed as the means ± SD; n=3 experiments, *p<0.01 vs. control, #p<0.05 vs. EtOH.
