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Tert-butylhydroquinone (tBHQ) prevents the ethanol (EtOH)-induced inhibition of antioxidant protein expression in H9c2 cells. Cell lysates were prepared from H9c2 cells cultured in control medium (control), exposed to 200 mM EtOH or tBHQ at 5 µM without EtOH, or exposed to EtOH and pre-treated with tBHQ (EtOH + tBHQ). The data are expressed as the means ± SD of 3 separate experiments; *p<0.05 vs. control, #p<0.05 vs. EtOH.
