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. 2016 May 9;36(1):10–22. doi: 10.3892/or.2016.4791

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of lncRNA-ATB on the cell cycle related-proteins. (A and C) Representative images showing the effect of lncRNA-ATB knockdown on the cyclin E, cyclin D1, p27 and p21 expression levels; (B and D) representative images showing the effect of lncRNA-ATB overexpression on the cyclin E, cyclin D1, p27 and p21 expression levels. After 4 days of transfection of lncRNA-ATB siRNA or lentivirus (LV)-mediated lncRNA-ATB plasmid in PC-3 and DU-145 prostate cell lines, the cells were lysed, and proteins from cell lysates were resolved by SDS-PAgE, transferred to a PVDF membrane blotted with anti-cyclin E, -cyclin D1, -p27, -p21 antibodies. Values are mean ± SD. ^*P<0.05 vs. Veh; ^✝P<0.05 vs. si-NC or LV-NC. n=4 for each group.