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. 2016 May 12;38(1):161–171. doi: 10.3892/ijmm.2016.2586

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Detection of apoptosis of renal cells by the TdT-mediated biotin-16-dUTP nick-end labeling (TUNEL) assay. The kidney sections were stained with 4′,6-diamidino-2-phenylindole (DAPI) in order to identify the nuclei. The rates of apoptosis in the positive control group confirmed the validity of the TUNEL assay results. (A) Apoptosis was significantly decreased in the hypothermic machine perfusion (HMP) group compared with that in the cold storage (CS) group (^**P<0.01). (B) A superimposed high power image. Nuclear counter staining by DAPI. (C) Quantitative analysis of apoptosis. ^*P<0.01. Results represent the means ± SD (n=5, magnification, ×200).