Figure 6.

miR-1 targets 3′-UTR of MET, cyclin D1 and cyclin-dependent kinase 4 (CDK4). (A) Conservation of the predicted miR-1 binding site in MET/cyclin D1/CDK4 3′-UTR across species. (B) Bioinformatics analysis of the interaction between miR-1 and MET, cyclin D1 and CDK4 3′-UTR binding sites. (C) Effect of miR-1 overexpression/downregulation on MET, cyclin D1 and CDK4 expression by western blot analysis, with GAPDH as the internal control. Proteins were quantified by densitometric analysis, which was performed using Quantity One software (Bio-Rad Laboratories). ^*P<0.05, ^#P<0.05.