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RASAL2 is a target of miR-136. (A) Sequence alignment of miR-136 and the 3′UTR of RASAL2 mRNA. (B) miR-136 overexpression repressed, whereas the ASO elevated the luciferase activities. (C) miR-136 mimics significantly decreased the mRNA level of RASAL2 and conversely, the ASO increased it (D). (E) Western blot analysis of RASAL2. Experiments were repeated as least three times independently. Values represent the mean ± SD. *P<0.05 was calculated using the Student's t-test.
