Figure 3.

Inflammatory responses induced by alkali burns are suppressed by diphenyleneiodonium (DPI) or apocynin (Apo). (A) Infiltration of inflammatory cells in corneas was detected by CD11b immunofluorescence staining. Green indicates the fluorescence signal of CD11b (original magnification, ×400). (B) CD11b/NOXs double-immunofluorescence staining was used to examine the distribution of Nox2, Nox4 and the inflammatory marker CD11b. Red and green indicate the fluorescence signals of CD11b and Nox2/Nox4, respectively. (C-E) mRNA levels of pro-inflammatory factors in corneas. RT-qPCR was applied to detect the gene transcription of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in mouse corneas. Three independent experiments for each condition were carried out. ^*P<0.05 vs. vehicle.