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. 2016 May 5;36(1):31–42. doi: 10.3892/or.2016.4786

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Copyright: © Seo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of quercetin on BT-474 cell growth. (A) BT-474 cells were treated with different doses of quercetin and genistein (0–100 µM). After 72 h, cell viability was assessed using a cell proliferation assay. (B) BT-474 cells were treated with different doses of quercetin (0–100 µM). The relative cell growth rate was measured by MTT assay after 24, 48 and 72 h. The growth rate of the vehicle-treated cells was set to 100%, and the relative decrease in cell viability resulting from the phytoestrogen treatment is expressed as a percentage of the control. (C) BT-474 cells were treated with different doses of quercetin (0–60 µM) for 72 h and photographed by phase-contrast microscopy (original magnification, x40). (D) BT-474 cells were treated with different doses of quercetin (0–20 µM). The relative cell growth rate was measured by MTT assay after 24, 48 and 72 h. Control cells were treated with DMSO alone. Data are shown as the means of three independent experiments (error bars denote SD). ^*P<0.05, ^**P<0.01, ^***P<0.001.

Effect of quercetin on BT-474 cell growth. (A) BT-474 cells were treated with different doses of quercetin and genistein (0–100 µM). After 72 h, cell viability was assessed using a cell proliferation assay. (B) BT-474 cells were treated with different doses of quercetin (0–100 µM). The relative cell growth rate was measured by MTT assay after 24, 48 and 72 h. The growth rate of the vehicle-treated cells was set to 100%, and the relative decrease in cell viability resulting from the phytoestrogen treatment is expressed as a percentage of the control. (C) BT-474 cells were treated with different doses of quercetin (0–60 µM) for 72 h and photographed by phase-contrast microscopy (original magnification, x40). (D) BT-474 cells were treated with different doses of quercetin (0–20 µM). The relative cell growth rate was measured by MTT assay after 24, 48 and 72 h. Control cells were treated with DMSO alone. Data are shown as the means of three independent experiments (error bars denote SD). ^*P<0.05, ^**P<0.01, ^***P<0.001.