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. 2016 May 5;36(1):31–42. doi: 10.3892/or.2016.4786

Figure 4.

Figure 4

Figure 4

Figure 4

Quercetin induces apoptosis via the extrinsic pathway in BT-474 cells. (A) BT-474 cells were incubated with quercetin (0–60 µM) for 72 h and were dyed with JC-1 (4 µg/ml). The data were analyzed by FACSCalibur flow cytometry measuring the green fluorescence and red fluorescence at 514/529 nm (FL-1) and 585/590 nm (FL-2), respectively. The data shown are representative of three independent experiments that gave similar results. (B and C) Analysis of intrinsic apoptosis-related molecules. BT-474 cells were treated with quercetin (0–60 µM) for 24 h. Total proteins were analyzed by western blotting with anti-Bcl-2, anti-BAX, anti-Bcl-xL and anti-tubulin antibodies.