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. 2016 May 5;36(1):31–42. doi: 10.3892/or.2016.4786

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Copyright: © Seo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of quercetin on STAT3 activation in BT-474 cells. (A) BT-474 cells were treated with quercetin (0–60 µM) for 24 h. Whole cell lysates were analyzed by western blotting with anti-p-p53, anti-p53, anti-p21 and anti-tubulin antibodies. (B) BT-474 cells were treated with quercetin (0–60 µM) for 24 h. Whole cell lysates were analyzed by western blotting with anti-p-JAK1, anti-p-STAT3, anti-STAT3, anti-VEGF, and anti-tubulin antibodies. (C) BT-474 cells were treated with quercetin (60 µM) for 24 h in the presence or absence of CoCl₂ (4 h). Whole cell lysates were analyzed by western blotting with anti-phospho-STAT3, anti-HIF-1α, anti-STAT3, and anti-tubulin antibodies. (D) BT-474 cells were treated with quercetin (60 µM) for 24 h in the presence or absence of CoCl₂ and then submitted to immunocytochemistry for detection of nuclear STAT3. The data shown are representative of three independent experiments that gave similar results. (E) BT-474 cells were transiently transfected with p4xM67-TK-luc plasmid containing four copies of the STAT-binding site, treated with quercetin (0–60 µM) and submitted to dual-luciferase assay. (F) BT-474 cells were treated with quercetin (0–60 µM) for 24 h and the intracellular MMP-9 concentration was measured by ELISA. Data are shown as the means of three independent experiments (error bars denote SD). ^*P<0.05, ^**P<0.01, ^***P<0.001.

Effect of quercetin on STAT3 activation in BT-474 cells. (A) BT-474 cells were treated with quercetin (0–60 µM) for 24 h. Whole cell lysates were analyzed by western blotting with anti-p-p53, anti-p53, anti-p21 and anti-tubulin antibodies. (B) BT-474 cells were treated with quercetin (0–60 µM) for 24 h. Whole cell lysates were analyzed by western blotting with anti-p-JAK1, anti-p-STAT3, anti-STAT3, anti-VEGF, and anti-tubulin antibodies. (C) BT-474 cells were treated with quercetin (60 µM) for 24 h in the presence or absence of CoCl₂ (4 h). Whole cell lysates were analyzed by western blotting with anti-phospho-STAT3, anti-HIF-1α, anti-STAT3, and anti-tubulin antibodies. (D) BT-474 cells were treated with quercetin (60 µM) for 24 h in the presence or absence of CoCl₂ and then submitted to immunocytochemistry for detection of nuclear STAT3. The data shown are representative of three independent experiments that gave similar results. (E) BT-474 cells were transiently transfected with p4xM67-TK-luc plasmid containing four copies of the STAT-binding site, treated with quercetin (0–60 µM) and submitted to dual-luciferase assay. (F) BT-474 cells were treated with quercetin (0–60 µM) for 24 h and the intracellular MMP-9 concentration was measured by ELISA. Data are shown as the means of three independent experiments (error bars denote SD). ^*P<0.05, ^**P<0.01, ^***P<0.001.