Table I.

Sequences of primers used for RT-qPCR.

Gene	Direction	Primer nucleotide sequence
Alkaline phosphatase (ALP)	Forward	5′-CATTGTGACCACCACGAGAG-3′
	Reverse	5′-CCATGATCACGTCAATGTCC-3′
Bone sialoprotein (BSP)	Forward	5′-ATTTTGGGAATGGCCTGTGC-3′
	Reverse	5′-GTCACTACTGCCCTGAACTGG-3′
Collagen type 1 (Col1A1)	Forward	5′-ACGAAGACATCCCACCAATC-3′
	Reverse	5′-AGATCACGTCATCGCACAAC-3′
Hypoxanthine-guanine phosphoribosyltransferase (HPRT)	Forward	5′-CCCTGGCGTCGTGATTAGTG-3′
	Reverse	5′-TCGAGCAAGACGTTCAGTCC-3′
Osteocalcin (OC)	Forward	5′-GGTGCAGCCTTTGTGTCC-3′
	Reverse	5′-TCAGCCAACTCGTCACAGTC-3′
Runt-related transcription factor 2 (RUNX-2)	Forward	5′-CGCCTCACAAACAACCACAG-3′
	Reverse	5′-ACTGCTTGCAGCCTTAAATGAC-3′