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. Author manuscript; available in PMC: 2016 Jun 8.
Published in final edited form as: Stroke. 2011 Nov 10;43(2):524–531. doi: 10.1161/STROKEAHA.111.635672

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

Identification of permeable cell types after intracerebral hemorrhage (ICH). (A-C) Detection of cell types with plasmalemma permeability to YOYO-1 iodide at 6 h after ICH. (A) Representative photomicrographs of YOYO-1+ cells (a), IBA-1+ (b), and overlay (c) showing no colocalization. (B) Representative photomicrographs of YOYO-1+ cells (d), GFAP+ cells (e), and overlay (f) showing no colocalization. (C) Representative photomicrographs of NeuN+ neurons (g) that colocalized with YOYO-1 (h) at 6 h after ICH (i, overlay) suggesting that neurons are particularly sensitive to plasmalemma damage early after ICH. (D) Detection of cell types with plasmalemma permeability to YOYO-1 at 24 h after ICH. By 24 h, YOYO-1+ cells (j) colocalized with IBA-1+ cells with morphological features of microglia (k) as shown in the overlay (l). YOYO-1+ cells (m) also colocalized with IBA-1+ cells with the morphological appearance of macrophages (n) at 24 h (o, overlay). Scale bar, 10 um for each panel.