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. 2016 May 16;9:1–14. doi: 10.1016/j.redox.2016.05.002

Fig. 4.

Fig. 4.

The NO release profiles of three different RSNOs and DETA/NO in different buffer conditions are shown. Ten milliliter of 50 μM CysNO, SNAP, GSNO and 25 μM DETA/NO prepared in PBS or DMEM were applied to the CellNO Trap for real-time monitoring, where panels A through D are CysNO, SNAP, GSNO, and DETA/NO, respectively. Under each condition, triplicate experiments were run independently. Data were presented as average (n=3) with error bar representing the standard deviation of the three trials. Panel (E) shows the NO release profile of 50 μM SNAP in 10 ml PBS. Since the release profiles of SNAP showed large variation among replicates, all repeats are presented for readers’ reference. Red marks indicate the highest flux values, red arrows show sharp decrease of the NO flux, green arrow, sharp increase. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)