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. 2016 May 16;9:1–14. doi: 10.1016/j.redox.2016.05.002

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 10. — Different media volumes illustrate an effect on the NO level experienced by the cultured cells at the bottom of the CellNO Trap. Panel (A), shows the NO release profiles of different volumes of 50 μM CysNO in DMEM with 5% CO₂; three independent experiments of each volume were run and data was presented as average of the three, error bar representing standard deviation. Panel (B) shows the change of the total NO and NO flux experienced by cells along with the change of the media volume, * and Δ indicate a significant difference of the total NO in between 5 ml and 10 ml, and between 5 ml and 15 ml respectively, P<0.05 by ANOVA and Tukey's test; star indicates a significant difference of the maximum NO flux between 5 ml and 15 ml, P<0.05 by ANOVA and Tukey's test.