Table 1.
Quantitative analysis of the NO release profiles of different NO donors.
| CysNO (50 μM ) | SNAP (50 μM ) | GSNO (50 μM ) | DETA NONOate (25 μM ) | |||
|---|---|---|---|---|---|---|
| Reportedt1/2(in PBS or PSS, h) | 0.023 | 1.15, 4.6, Up to 6 | 159 | 20 | ||
| PBS | w/out CO2 | Total NO (mol) | 7.37E-08 (4.76E-09) | 8.87E-08 (4.87E-08) | 1.09E-07 (6.30E-08) | 9.30E-08 (1.30E-08) |
| Average flux (mol/cm2/s) | 2.49E-12 (1.61E-13) | 4.89E-13 (2.92E-13) | 9.06E-14 (5.21E-14) | 8.11E-14 (1.14E-14) | ||
| Max flux (mol/cm2/s) | 2.12E-11 (1.89E-12) | 1.11E-12 (6.72E-13) | 4.36E-13 (4.80E-13) | 1.19E-13 (1.32E-14) | ||
| Duration (h) | 0.62 (0.024) | 4.04 (2.76) | >24 h* | >24 h* | ||
| DMEM | w/out CO2 | Total NO (mol) | 1.38E-08 (8.88E-10) | 1.18E-07 (2.96E-09) | 1.36E-07 (2.70E-08) | 8.44E-09 (3.94E-09) |
| Average flux (mol/cm2/s) | 5.76E-13 (3.70E-14) | 6.96E-13 (2.86E-14) | 5.68E-13 (1.25E-13) | 7.22E-15 (3.30E-15) | ||
| Max flu (mol/cm2/s) | 2.65E-12 (1.08E-12) | 3.05E-13 (7.67E-15) | 1.24E-12 (2.22E-13) | 2.55E-14 (1.14E-14) | ||
| Duration (h) | 0.45 (0.056) | 6.42 (0.12) | 22.01 (0.83) | >24 h* | ||
| with CO2 | Total NO (mol) | 7.43E-08 (1.14E-09) | 1.10E-07 (2.78E-08) | 1.64E-07 (4.94E-09) | 1.55E-07 (2.30E-08) | |
| Average flux (mol/cm2/s) | 5.07E-13 (1.49E-14) | 7.11E-14 (8.50E-15) | 1.44E-13 (6.42E-15) | 1.32E-13 (1.96E-14) | ||
| Max flux (mol/cm2/s) | 1.73E-12 (6.81E-14) | 1.25E-13 (1.41E-14) | 4.31E-13 (1.59E-14) | 2.33E-13 (3.39E-14) | ||
| Duration (h) | 2.44 (0.46) | >24 h* | 4.48 (0.80) | >24 h* | ||
The total moles of NO were calculated by integrating the area under the NO release curves; maximum NO flux was directly read from the NO releasing profile; average NO flux refers to the entire releasing duration; t1/2 is the half-life of donors; * duration is longer than 24 h, however we only reported the initial 24 h. Data is presented as the average and (Std. dev.).