Table 2.
Quantitative analysis of transient ion effect on RSNO's NO generation.
| CysNO (50 μM ) | SNAP (50 μM ) | GSNO (50 μM ) | ||
|---|---|---|---|---|
| PBS | First 10 min NO release(mol) | 6.64E-08 (4.12E-09) | 4.69E-09 (2.32E-09) | 2.13E-09 (2.07E-09) |
| PBS with EDTA | Total NO (mol) | 1.12E-09 (2.41E-10) | 1.53E-09 (5.97E-10) | 3.53E-11 (7.80E-12) |
| Average flux (mol/cm2/s) | 2.97E-14 (6.75E-15) | 8.88E-15 (2.06E-15) | 5.43E-14 (2.84E-14) | |
| Max flux (mol/cm2/s) | 1.64E-13 (2.42E-14) | 1.46E-14 (3.22E-15) | 8.99E-15 (1.71E-15) | |
| First 10 min NO release (mol) | 4.74E-10 (1.07E-10) | 8.24E-11 (2.49E-11) | 2.05E-10 (1.47E-10) | |
| Ratio (with TIs/without TIs) | 140.1 | 56.9 | 10.4 | |
NO moles were calculated by integrating the area under NO releasing curve between 2 specific time points; first 10 min was chosen because in PBS solutions CysNO's NO releasing in PBS and all RSNOs' releasing in 10 mM EDTA PBS became almost undetectable. Data is presented as the average and (Std. dev.).