Table 5.
Quantitative analysis of cell culture NO profiles treated by soluble NO donors.
| CysNO (50 μM ) | SNAP (50 μM ) | GSNO (50 μM ) | DETA/NO (25 μM ) | |||
|---|---|---|---|---|---|---|
| Complete media with cells | Collagen I coating with cells | Total NO (mol) | 1.67E-07 (5.17E-08) | 1.71E-07 (2.39E-08) | 1.32E-07 (2.15E-08) | 2.54E-08 (4.21E-09) |
| Average flux (mol/cm2/s) | 1.07E-12 (2.56E-13) | 3.77E-13 (8.33E-14) | 4.70E-13 (3.35E-14) | 3.21E-14 (1.05E-14) | ||
| Max flux (mol/cm2/s) | 4.96E-12 (1.18E-12) | 5.92E-13 (7.42E-14) | 1.80E-12 (3.58E-13) | 7.38E-14 (3.40E-15) | ||
| Duration (h) | 3.15 (0.98) | 9.46 (2.55) | 4.44 (0.43) | 14.60 (3.12) | ||
| Dopamine coating with cells | Total NO (mol) | 1.30E-07 (3.15E-08) | 1.87E-07 (4.92E-08) | 1.31E-07 (2.60E-08) | 9.30E-08 (3.20E-08) | |
| Average flux (mol/cm2/s) | 6.86E-13 (4.68E-13) | 4.61E-13 (9.00E-14) | 5.95E-13 (1.05E-13) | 1.38E-13 (7.42E-14) | ||
| Max flux (mol/cm2/s) | 2.67E-12 (5.46E-13) | 8.40E-13 (2.94E-14) | 1.24E-12 (3.85E-13) | 2.48E-13 (9.03E-14) | ||
| Duration (h) | 2.76 (0.19) | 5.33 (2.20) | 4.06 (0.64) | 13.15 (1.06) | ||
Cells were cultured in either a collagen I top-coated or a dopamine-gelatin treated CellNO Trap device, respectively. NO donors were dissolved in media to twice the desired final concentration in equal volume media to original cell culture media and applied to the cultured cells. Data is presented as the average and (Std. dev.).