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. Author manuscript; available in PMC: 2017 Jun 21.
Published in final edited form as: Analyst. 2016 Mar 23;141(12):3648–3656. doi: 10.1039/c6an00200e

Figure 2.

Figure 2

Enhancing small-molecule detection from single blastomeres using multi-solvent extraction. (A) The polarpH4, apolarpH5, and apolarpH8 solvents allowed us to detect a complementary set of small molecules in the cells. Numbers indicate different compounds. (B) These solvents also provided complementary performance in electrophoretic separation; 28 select small molecules are shown. Higher signal-to-noise ratio and separation power was achieved using apolarpH5, and apolarpH8 solvents. Theoretical plate numbers (N) are provided for arginine. Key: CR, creatine; GSH, glutathione; GSSG, oxidized glutathione; HPX, hypoxanthine; S-adenosylmethionine (9); ornithine (10); lysine (11). Identified compounds are listed in Table S2.