Table 4.

Cell expansion medium composition (blank entries indicate the information was not available to the authors). The last column represents additional supplements that were added to the existing expansion medium to formulate the medium used for 3D culture. Cell passage numbers are indicated in Table 1.

Product	Base Medium	Serum	Antibiotics	Growth Factors	Ascorbate	Other Additives	3D Culture
Biocart™II		Autologous serum		FGF-2 variant (10 ng/mL)
Bioseed-C		Autologous serum
Cartipatch®	DMEM/Ham’s F-12 (1:1)	10% autologous serum	Gentamycin fungizone (discontinued after P1		50 μg/mL
Chondrosphere®	DMEM/Ham’s F-12 (1:1)	Autologous serum	None	None
Hyalograft® C	Ham’s F-12	10% FCS	Penicillin streptomycin	TGF-B1 (1ng/mL), FGF-2 (5ng/mL), EGF (1ng/mL)		1 ng/mL insulin 1% L-glutamine	+ 50 mg/mL ascorbic acid
MACI	DMEM	9% FBS	Gentamycin
NeoCart®
NOVOCART® 3D		Allogeneic serum	None
RevaFlex™	HL-1 or similar	10% serum to serum-free	Yes		50 μg/mL