Figure 1. FMD cycles decrease disease severity of the MOG35-55-induced EAE model.
(mean ± S.E.M, * p < 0.05, ** p<0.01; *** p < 0.001; t test, 1-way or 2-way ANOVA & Bonferroni Post Test; Scale bar represents 200 μm).
a. Diagram displaying the time course of the immunization and the diet interventions.
b. The EAE severity scoresof the Control Diet (EAE CTRL; n=23), ketogenic diet (EAE KD); n=13, semi-therapeutic FMD cycles (EAE FMD(S); n=7) or therapeutic FMD cycles (FMD(T); n = 23).
c. Incidence rate of the EAE CTRL and EAE FMD (S) (n=7–23).
d. EAE severity score of the EAE FMD(T) mice that completely reversed the EAE severity and scored 0, no observable disease (n=5).
e. EAE severity score of the best-performing control mice (n=12) and the FMD(T) mice (n=12).
f. EAE severity score of the EAE CTRL mice that treated with FMD upon chronic EAE development(EAE CTRL-FMD) (n=6).
g–m. Spinal cord of the EAE CTRL and the EAE FMD (T) mice with quantification of (g) H&E staining, (h) solochrome cyanine staining, and (i) MBP (Myelin Basic Protein) and SMI32 staining of spinal cord sections isolated at day 14.