Figure 3. Antigen activated splenocytes from the EAE-CTRL and the EAE-FMD mice had similar encephalitogenic effects.
(n=5–6 / group; mean ± S.E.M, * p < 0.05, ** p<0.01; *** p < 0.001; t test, 1-way ANOVA & Bonferroni Post Test).
a. Diagram for the adoptive transfer EAE model.
b–d. Quantification of the (b) TNFα, (c) IFNγ, and (d)IL-17 (pg/mL) of the supernatant from ex-vivo culture of splenocytes from naïve, EAE CTRL, and EAE FMD either with or without MOG35-55 and IL-23 re-activation
e–f. Quantification of TH1 or TH17 (represented by % of CD4+) from lymphocytes culture of EAE CTRL and EAE FMD with or without MOG35-55 and IL-23 re-activation
g. Incidence rate of adoptive transfer EAE groups.
h. EAE severity score of adoptive transfer EAE groups.