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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Transfusion. 2016 Apr 4;56(6):1362–1369. doi: 10.1111/trf.13595

Table 2. Percentage of RBCs expressing lactadherin and CD47 expression in mean fluorescent intensity (MFI) on transfused, untransfused and in vitro biotin labelled RBCs.

Before Transfusion After Transfusion

Unlabeled and untransfused RBCs sampled from circulation BioRBCs only Unlabeled RBCs from circulation BioRBCs isolated from circulation
Membrane bound Lactadherin (%) Control 1.10 (0.07-1.43) N/A 1.10 (0.67-1.73) N/A
2D RBCs 0.40 (0-2.02) 1.10 (0.05-1.82) 0.40 (0.00-2.65) 1.85 (0.84-3.26)
35D RBCs 0.60 (0.03-1.29) 0.63 (0-5.32) 0.55 (0.12-1.30) 0.30 (0.00-1.06)

CD47 (MFI) Control 2383 (2091-2446) N/A 1998 (1766-2088) N/A
2D RBCs 1582 (890-1820) 1690 (1360-1951) 1582 (1001-1948) 1542 (1196-2106)
35D RBCs 1309 (1161-1567) 1599 (1533-1666) 1309 (1180-1684) 1497 (1313-1817)
*

The column “Before Transfusion” describes the background lactadherin and CD47 expression on naïve RBCs drawn from the circulation and BioRBCs directly after labeling. The column “After Transfusion” describes the sample which has been drawn after transfusion from which both BioRBCs and unlabeled RBCs have been isolated. Values are expressed as medians and confidence intervals between brackets.