Skip to main content
. Author manuscript; available in PMC: 2016 Jun 9.
Published in final edited form as: Cell Rep. 2016 May 26;15(10):2159–2169. doi: 10.1016/j.celrep.2016.04.087

FIGURE 1. Enhancer hubs and their impact on super-enhancer activity.

FIGURE 1

(A) Top: Scheme showing the location of the Igk AgR locus with its respective enhancers: MiEκ, 3’Eκ and Edκ on murine chromosome 6. Bottom: Outline of the different stages of B cell development. Stages under investigation are highlighted in orange (pre-B and immature B). (B) Left: Distribution of H3K27Ac signal across the peaks identified by MACS in pre-B and immature B cells with super-enhancers containing an exceptionally high amount of H3K27Ac. Right: H3K27Ac signal at the 3’ end of Igk in pre-B and immature B cells with the region defined as the super-enhancer highlighted. ATAC-seq profiles of the region in wild-type pre-B cells. (C) Detailed scheme showing the location of MiEκ and 3’Eκ 4C baits. (D) 4C signal normalized by DESeq2 in 5kb windows sliding by 0.5kb for ~50kb region neighboring the MiEκ and 3’Eκ baits in WT versus enhancer-deficient cells. Filled circles highlight significant differences in 4C-seq counts identified by DESeq2 analysis of the plotted region. Transcriptional output within the region is represented below each plot by RNA-seq profiles. (E) Model showing the organization of the individual enhancer elements within the Igk super-enhancer in wild-type versus MiEκ−/− and 3’Eκ−/− pre-B cells. See also Figure S1.