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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Eur J Neurosci. 2016 May 10;43(11):1509–1522. doi: 10.1111/ejn.13243

Fig. 2. Localization and expression of mGluR6 and TRPM1 may be only mildly affected.

Fig. 2

A. Immunostaining for mGluR6 in WT and Gnao1-KO as indicated. B. Immunostaining for TRPM1 in WT and KO as indicated. C. Average staining for TRPM1 in OPL and INL was not significantly different between WT and KO. D. Western blots for mGluR6 and actin (top) and TRPM1 and actin (bottom) for WT and KO as indicated. E. Quantification of Western blots for mGluR6 and TRPM1. Total band intensity for mGluR6 (or TRPM1) was divided by that for actin in each sample (three replicates per sample, sample was taken from 1–2 retinas from each mouse) and then averaged across samples to yield relative intensity (n=4 mice for mGluR6 and n=3 mice for TRPM1).