Table 1. LptDE data collection and refinement statistics.

	Yersinia LptDE	Klebsiella LptDE	Full-length Klebsiella LptDE	Pseudomonas LptDE
Data Collection
wavelength (Å)	1.0	1.0	1.0	1.0
Space Group	P21	P21	I222	C2221
Mol/ASU‡	4	2	1	1
X-ray source	APS-22-ID	APS-22-ID	APS 23-ID-D	APS 23-ID-B
a, b, c (Å)	82.8, 176.3, 143.8	75.2, 173.0, 84.0	130.6, 154.9, 194.9	149.1, 156.1, 115.8
α, β, γ (°)	90, 96.1, 90	90, 111.3, 90	90, 90, 90	90, 90, 90
dmin (Å)*	2.75 (2.85-2.75)	2.95 (3.06-2.95)	4.45 (4.57-4.45)	3.00 (3.11-3.00)
Completeness (%)*	99.3 (95.1)	97.4 (95.1)	95.6 (94.7)	100.0 (100.0)
Redundancy*	3.6 (3.4)	4.0 (3.8)	5.5 (4.9)	7.2 (7.1)
Rmerge †*	0.159 (0.786)	0.067 (0.999)	0.099 (0.848)	0.132 (0.999)
I/σ (I)*	8.4 (1.48)	16.6 (1.34)	14.3 (1.8)	12.4 (1.25)
Refinement
Resolution	40.1-2.75	41.4-2.95	44.7-4.36	49.0-2.98
Reflections	106218	41834	10921	27493
Atoms	22831	11347	7275	6104
Amino acids	2740	1566	908	731
Rwork§/Rfree¶	0.21/0.26	0.23/0.28	0.30/0.34	0.24/0.29
PDB code	5IXM	5IV8	5IV9	5IVA
Validation
Average B-factor (Å2)	47.0	72.6	97.1	81.2
Bond angle RMSD (°)	0.998	0.488	0.631	0.668
Bond length RMSD (Å)	0.004	0.002	0.003	0.003
Ramachandran favored (%)¥	91.5	93.5	86.8	92.5
Ramachandran allowed (%)¥	6.2	5.9	10.1	6.8
Clashscore	9.7	2.7	5.4	7.2

^‡Copies of the LptDE complex per asymmetric unit.

^*Indicates statistics for last resolution shell shown in parenthesis.

^†R_merge = Σ_hkl,j (|I_hkl-<I_hkl>|) / Σ_hkl,j I_hkl, where <I_hkl> is the average intensity for a set of j symmetry related reflections and I_hkl is the value of the intensity for a single reflection within a set of symmetry-related reflections.

^§R_work = Σ_hkl (||F_o| - |F_c||) / Σ_hkl|F_o| where F_o is the observed structure factor amplitude and F_c is the calculated structure factor amplitude.

^¶R_free = Σ_hkl,T(||F_o| - |F_c||) / Σ_hkl,T|F_o|, where a test set, T, is omitted from the refinement.

^¥Calculated with Molprobity.