. Author manuscript; available in PMC: 2017 Jul 1.

Published in final edited form as: Arch Biochem Biophys. 2016 Feb 23;601:88–96. doi: 10.1016/j.abb.2016.02.023

Table 2.

Effect of Cardiac Troponin I C-terminal deletions on speed-pCa relations in motility assays.

Troponin Complex	Regression parameter estimates ± SE
Troponin Complex	pCa₅₀	n_H	S_min/(S_min + S_max)	R²
WT Tn	5.89 ± 0.01	12.7 ± 2.1	0	0.981
Tn containing cTnI Δ1	5.95 ± 0.01	7.1 ± 0.4	0	0.999
Tn containing cTnI Δ3	6.08 ± 0.02	(5.5)	0.04 ± 0.02	0.987
Tn containing cTnI Δ5	6.51 ± 0.03	3.9 ± 1.2	0.07 ± 0.05	0.947

Regression parameter estimates for motility data shown in Fig. 5 were obtained as described in Materials and Methods. Note that n_H for Tn containing cTnI Δ3 was constrained to be 5.5 because the data did not adequately define this slope parameter.