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. Author manuscript; available in PMC: 2016 Oct 7.
Published in final edited form as: Anal Chem. 2016 May 23;88(11):5725–5732. doi: 10.1021/acs.analchem.5b04858

Figure 1.

Figure 1

Elution time of intact glycopeptides versus non-glycosylated peptides. The distributions of the elution time shift between intact glycopeptides and non-glycosylated peptides (in de-glycolated samples) manually identified in breast cancer cell line MDA-MB-453 (a) and MDA-MB-361 (b) show that most intact glycopeptides elute within a fixed window (i.e., of 4~14 minutes) ahead of the corresponding de-glycosylated peptides, although there exist some intact glycoglycopeptides eluting at the time later than the elution time of the corresponding deglycosylated peptides. We note there were exceptional cases (one in each of these two samples, respectively) that are not shown in the distribution, where one intact glycopeptide elute far later (12 minutes and 30 minutes, respectively) than the corresponding deglycosylated peptide, perhaps due to false peptide. (c–d) Extracted ion chromatogram (XIC) of an intact glycopeptide and non-glycosylated peptide of AEPPLNASASDQGEK (c) and AAIPSALDTNSSK (d).