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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Endocr Relat Cancer. 2016 Apr 29;23(5):L13–L16. doi: 10.1530/ERC-16-0148

Figure 2.

Figure 2

A. Immunoprecipitation of HIF-2α ubiquitination. Recombinant HIF-2α protein was immunoprecipitated with an antibody against HA-tag. Protein ubiquitination was probed with a polyclonal antibody against ubiquitin. B. CHX chase assay measurement of HIF-2α protein stability. C. Quantification of HIF-2α protein stability on the basis of data shown in B. D. Quantitative mRNA expression measurement of four hypoxia-related genes in tumor specimen from patient 1: EDN1, EPO, GLUT1, GNA14, LDHA, and VEGFA – performed by qPCR assay. A normal adrenal medulla (NM) specimen was used as a control.