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. Author manuscript; available in PMC: 2016 Jun 9.
Published in final edited form as: Cell Rep. 2016 May 26;15(10):2127–2135. doi: 10.1016/j.celrep.2016.05.004

Figure 1.

Figure 1

Mis18BP1 N-terminus and CBD domain are required for centromere localization. (A) Schematic of GFP-tagged Mis18BP1 constructs (B) GFP-Mis18BP1 constructs expressed in U2OS cells. Centromeres (CEN) were identified using anti-CENP-A (Mis18BPΔSANTA) or anti-CENP-T antibodies. Scale bar, 5 μm. (C) Quantitation of GFP centromeric localization of Mis18BP fragments. ± S.D., * p < 0.05. (D) CENP-A recruitment to centromeres in U2OS cells treated with siRNA against GAPDH or Mis18BP1 and rescued by transfections with GFP-Mis18BP12-721 or control plasmid (myrGFP) (E) Relative CENP-A intensity in cells treated with Mis18BP1siRNA and rescued with GFP-Mis18BP1N→CBD. See also Figure S1.