Figure 2.

Structure-based sequence alignment. Elements of secondary structure are designated above the sequence as cartoon shapes. Blue text highlights positions that are completely conserved across all nine structures. A black dot above the sequence alignment designates a position which contacts the bound DNA in at least one of the nine structures. The inset box provides the DNA target sequence for each meganuclease, with the central four basepairs highlighted red. See also Figures S2 and S3, which provides a corresponding ‘contact map’ of all superimposable positions on the nine protein backbones that are involved in DNA contacts.