Table 1.

Primers used for amplification, cloning, and sequencing of variable regions of m864 for use in construction of 864-cIgG

Primer	Use	Sequence
MHV9 5′	Heavy chain variable region amplification	act agt cga cat ggm ttg ggt gtg gam ctg cta ttc ctg
MHV2a 3′	Heavy chain variable region amplification	att cgg ata gat cta gtg gat aga ccg atg g
MKV4 5′	Light chain variable region amplification	act agt cga cat gag grc ccc tgc tca gwt tyt tgg mwt ctt g
MKV5 5′	Light chain variable region amplification	act agt cga cat gga ttt wca ggt gca gat twt cag ctt c
MKV REV 3′	Light chain variable region amplification	att cgg ata gat ctt gga tgg tgg gaa gat g
h864 Hv dEcoRI 5′	Heavy chain variable region cloning	cct gcg gca gaa ttc ggt tca
h864 Hv dNheI 3′	Heavy chain variable region cloning	gac tag gta gct agc tga gga
h864 Kv dEcoRI 5′	Light chain variable region cloning	ctg cgg caa gaa ttc aat gac
h864 Kv dBsiWI 3′	Light chain variable region cloning	cct gcg gca cgt acg ttt gat
pFUSE hG1 Seq 5′	Heavy chain variable region sequencing	tct cca cgc ttt gcc tga ccc tg
pFUSE hG1 Seq 3′	Heavy chain variable region sequencing	ggg cgc ctg agt tcc acg aca ccg
pFUSE hK Seq 5′	Light chain variable region sequencing	ccc ttg gag cct acc tag act cag ccg
pFUSE hK Seq 3′	Light chain variable region sequencing	cca cct tcc act gta ctt tgg cct ctc tg