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. Author manuscript; available in PMC: 2016 Oct 25.
Published in final edited form as: Nat Struct Mol Biol. 2016 Apr 25;23(6):531–539. doi: 10.1038/nsmb.3212

Figure 6. The CVSC subunit pUL25 depends on pUL17 to bind capsids.

Figure 6

(a) At top, Western blots probing for pUL25 and pUL17 in nucleocapsids of wild-type HSV-1 KOS, UL25-null and UL17-null mutants. At bottom, SDS-PAGE of the capsid samples with bands identifying the major capsid protein as marked: VP5, triplex subunits VP19C and VP23, and the hexon capping protein VP26. (Complete images are available in Supplementary Data Set 1.) (b) Central section through cryoEM reconstructions of the HSV-1 null mutant capsids, as marked. The triplex molecule is indicated by “T”, and the penton by “P”. (c) Corresponding sections through reconstructions of native capsids imaged inside intact virions of HSV-1 and PRV, as marked. Scale bar 100Å.