Skip to main content
. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Leuk Res. 2016 Apr 16;46:51–60. doi: 10.1016/j.leukres.2016.04.006

Figure 4. Pknox1 is a valid target of the miR-17~92 cluster.

Figure 4

A) Putative miRNA response element mutations in PKNOX1 3′UTR. Nucleotide changes for mutant constructs are indicated with underlines. B) Schematic of luciferase reporter construct design and mutation placement. C) Luciferase response relative to wild type control. Luciferase levels were examined in 4 independent experiments. Statistical significance was tested against wt (p< .05) using student’s t-test. D) Endogenous levels of PKNOX1 and PBX1/2/3 were examined in human cell lines. Unnecessary lanes cropped out.