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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Leuk Res. 2016 Apr 16;46:51–60. doi: 10.1016/j.leukres.2016.04.006

Figure 5. Antagomir treatment results in increased PKNOX1 levels and participation in HOX-PBX complexes.

Figure 5

A) PKNOX1 protein levels in HEK293T cells were measured in response to transfection with increasing concentrations of a plasmid expressing miR-17~19b with quantification of PKNOX1 levels relative to loading control indicated below blot. B) Human leukemia cell lines were treated with antagomirs (2 μM for 72 hrs) and analyzed for PKNOX1 protein levels by western blot. B) HEK293T cells were transfected with Meis1 expressing plasmid and subjected to co-immunoprecipitation for PBX1/2/3. 20% input (bottom) was subject to western blotting for PKNOX1 and MEIS1. Co-immunoprecipitation was performed using antibody against PBX1/2/3. Representative blot depicted from experiment were performed in triplicate.