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. 2016 Jun 9;6:38. doi: 10.1186/s13568-016-0210-3

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — SAM production mechanism of high-SAM-producing strain estimated in this study. a Without methionine. b With methionine. c With methionine and without yeast extract. The dotted gray arrows represent respiration. The metabolome analysis results revealed that the rate-limiting step for SAM production without and with methionine was methionine and ATP supply, respectively (gray characters). Higher SAM production without yeast extract was explained by enhanced ATP supply with inhibited cell growth