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. 2016 May 20;5(1):671. doi: 10.1186/s40064-016-2318-y

Fig. 4.

Fig. 4

Amplification plots of the lyophilization assay. Fresh positive control showed a Cycle threshold (Ct) of 22, trehalose 0 % Ct of 24, trehalose 2.5 % Ct of 26, trehalose 5 % Ct of 29 and trehalose 10 % Ct of 33