Fig. 9.

HSV-1 reactivation from infected mice and tree shrews brain. Mice and tree shrews were infected with HSV-1 strain McKrae at the indicated dose via eye cornea inoculation. At 30 days post-infection (dpi), neural tissues were harvested and assayed for reactivation of latent virus as described in the “Methods.” Data were collected from at least two independent experiments. The number of animals assayed in each group was 18. a Olfactory bulb, blue rhombus represented mouse (11 % reactivation). Orange square represented tree shrew olfactory bulb (22 % reactivation). b Brain stem, blue triangle represented mouse (67 % reactivation); orange circles represented tree shrew (11 % reactivation). c Viral DNA load in latent mouse and tree shrew olfactory bulbs and brain stems. Brain stems and olfactory bulbs of tree shrews or mice infected with HSV-1 McKrae for 30 days were dissected to quantify the numbers of copies of viral genomes per μg of tissue DNA. Each point on the scattergrams represents an individual sample, and the horizontal lines represent the mean values for each group. *P < 0.05; Mann–Whitney U test