Table 1.

PCR primer sets used to characterize B. fragilis and BFT gene isotypes.

Primer name	Primer sequence (5' →3')	Reference strain / gene access	Bacterial target	Gene	Reference(s)
		CR626927.1, AP006841.1, NC_016776.1	B. fragilis	leuB	[37]
Leu-3	CACTTGACTGTTGTAGATAAAGC
Leu-4	CATCTTCATTGCAGCATTATCC
Leu-Prb	TGTGCTTGCTTCCAGTCGTCTATG
Bf904F	GGCGGTCTTCCGGGTAAA	GU130198; GU130199; GU130200, CR626927	B. fragilis	gyrB	[32, 38]
Bf958R	CACACTTCTGCGGGTCTTTGT
Bf923-MGB	FAM-TGGCCGACTGCTC-MGBNFQ
qHS601F	GTTGTGAAAGTTTGCGGCTCA	ATCC25285/CR626927	B. fragilis	16S rRNA	[33, 39]
qBAc725R	CAATCGGAGTTCTTCGTGATATCTA
qHS624MGB	FAM-CGTAAAATTGCAGTTGA-MGB
F	TCRGGAAGAAAGCTTGCT	ATCC25285/CR626927	B. fragilis	16S rRNA	[31]a
R	CATCCTTTACCGGAATCCT
Prob	ACACGTATCCAACCTGCCCTTTACTCG
bftF	GGG ACA AGG ATT CTA CCA GCT TTA TA	VPI 13784 (bft-1),   86-5443-2-2 (bft-2)	ETBF	bft	[36, 40]
bftR	ATT CGG CAA TCT CAT TCA TCA TT
bftProb	CGC AAT GGC GAA TCC ATC AGC TAC A
bft-1F	GGG ATG TCC TGG TTC A	AB026625	ETBF	bft-1	[36, 40]
bft-1R	AAT TAT CCG TAT GCT CAG CG
bft-1Prob	CTT CGG ATT TTR AAG CCA GTG GGA TGT C
bft-2F	CTT AGG CAT ATC TTG GCT TG	AB026626	ETBF	bft-2
bft-2R	GCG ATT CTA TAC ATG TTC TC
bft-2Prob	CTT CGG ATT TTR AAG CCA GTG GGA TGT C
bft-3F	TTT GGG CAT ATC TTG GCT CA	AB026624	ETBF	bft-3
bft-3R	ATC ATC CGC ATG GTT AGC A
bft-3Prob	CTT CGG ATT TTR AAG CCA GTG GGA TGT C

^aPCR primers/probes to detect other members in B. fragilis group were also described in this report.