Table 4.

Identification of S. aureus, including MRSA, using multiplex RT-PCR.

Method	Gene	Genus	Specimen	Sensitivity	Specificity	Time	Reference
Multiplex real time PCR	SCCmec/orfX junction; lukF and lukS	MRSA	nasal swabs	95%, 93.5%	99%, 82.9%	NA	[48, 50]
Multiplex real time PCR	various SRE sequences, orfX(Xsau325), SCCmec	MRSA	clinical specimens	NA	NA	<1 h	[44, 45]
Triplex real time PCR	mecA, coa, Sa442 , ermA,femA	S. aureus and methicillin resistance	Clinical isolates	100%	100%	3h	[43, 47]
Triplex real time PCR	tuf, nuc, and mecA	S. aureus	blood culture	99.2%-100.0%	98.7%-100.0%	90 min	[51]
Double triplex real time PCR	tuf, nuc, mecA, atlE, gap and mvaA	S.aureus	blood culture	NA	NA	83 min	[52]