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Expression levels of FSHβ (A, B) and LHβ (C, D)mRNAs in Nile tilapia pituitaries cultured with different concentrations of NKB (A, C) or NKBRP (B, D) peptides. Relative abundance of the mRNAs was normalized to the amount of GAPDH by the comparative threshold cycle method using qRTPCR. Results are means ± SEM (n=5–8). * indicates significant difference from 0 μM group (P<0.05).
