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. 2016 Mar;20(1):51–61. doi: 10.12717/DR.2016.20.1.051

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — Expression levels of GnRH I (A), Kiss2 (B) andtac3 (C) mRNAs in the brains and FSHβ (D) and LHβ (E) mRNAs in the pituitaries of Nile tilapia injected either with LHRH, NKB, NKBRP or PBS. Relative abundance of the mRNAs in the brains was normalized to the amount of GAPDH and β-actin and in the pituitaries was normalized to the amount of GAPDH by the comparative threshold cycle method using qRT-PCR. Results are means ± SEM (n=6–8). * indicates significant difference from PBS group (P<0.05).