Table 1.

Primers used for quantitative real-time PCR(qRT-PCR)

	Sequence	Product size
Tac3 F	5'-CTT ATG GGA CGG AGA AAC GC-3'	96 bp
Tac3 R	5'-CCT CAG CCT GCA CTT GTT GA-3'
LHβ F	5'-GCT GTC ACC CAG TAG AGA-3'	79 bp
LHβ R	5'-TTG CTG AAT GGT ATC TTG ATG A-3'
GnRH I F	5'-CTC GCA GGG ACG GTG TTT-3'	70 bp
GnRH I R	5'-TCT TCC CTC CTG GGC TCA GT-3'
FSHβ F	5'-GGC TTC GTC GAC ACC ACC AT-3'	148 bp
FSHβ R	5'-TGA AAC CCC GTG GAC ATT GC-3'
Kiss2 F	5'-CTA CTG GCT TTG GCT GTG GT-3'	112 bp
Kiss2 R	5'-CTG CTC CTG TTG CAT GTG TT-3'
GAPDH F	5'-TTA AGG AAG CCG TCA AGA AG-3'	126 bp
GAPDH R	5'-CAG CAC CAG CAT CAA AGA-3'
β-actin F	5'-AGC ATC CCG TCC TGC TCA CA-3'	121 bp
β-actin R	5'-AGC ACA GCC TGG ATG GCA AC-3'