Figure 3.

Sensitivity of RPA mediated target amplification. Duplex assays were performed with primers specific for endogenous lec (right panel) and RR2Y target (left panel) in the same reaction. Reactions without template DNA (NTC) were also carried out as a control. (a) Amplification curves of RPA reactions using DNA template at 100, 25, or 10 copies per reaction. (b) Amplification curves of RPA reactions using 25 or 13 copies of RR2Y DNA template per reaction in the presence of large amounts of conventional soybean DNA.