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. 2016 May 26;2016:1290561. doi: 10.1155/2016/1290561

Figure 2.

Figure 2

Only high nestin-expressing mDPSC clones possess the ability to differentiate into neuronal-like cells. (a) Representative phase contrast images of high (clone 2) and low (clone 3) nestin-expressing clones prior to and following 15 days of neuronal differentiation demonstrating a more neuronal-like morphology in high nestin-expressing clones with small refractive cell somas extending multiple interconnecting processes. (b) Immunocytochemical staining identified the presence of microtubule-associated protein 2 (Map2) and NF-l in high, but not low, nestin-expressing mDPSC clones following 15 days of neuronal differentiation. (c) Changes in mRNA expression of mesenchymal and neural markers during neuronal differentiation of high nestin-expressing mDPSC clones (clone 2). Expression levels of target genes were normalized against GAPDH and the 2−ΔΔCT method for qPCR analysis used to calculate fold change in expression relative to predifferentiation cells on day 0 ± SEM (n = 3 independent differentiation experiments). One-way ANOVA with Dunnett multiple comparisons posttest to identify significant increases/decreases in expression compared to day 0 cells: ∗ p < 0.05 and ∗∗ p < 0.01. Scale bars = 100 μm.