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. 2016 May 26;2016:1290561. doi: 10.1155/2016/1290561

Figure 3.

Figure 3

Neuronally differentiated high nestin-expressing mDPSCs show immature electrophysiology properties. Current density-voltage relationships of transmembrane K+ currents of undifferentiated (a) and neuronally differentiated (b) high nestin-expressing mDPSCs (clone 2) in the absence (○, inlet 1 illustrates exemplar trace of currents, n = 14  and  17 cells, resp.) and presence of TEA (1 mM) (■, inlet 2 illustrates exemplar trace of currents, n = 9 for each differentiation condition). (c) Current density-voltage relationships of transmembrane K+ current (○) and Na+ currents (▼) of mSTM neurons (n = 23 cells). Inlet illustrates exemplar trace of currents. The mean ± SEM current densities at +80 mV of undifferentiated (6.4 ± 1.5 pA/pF) and neuronally differentiated mDPSCs (7.3 ± 1.4 pA/pF) showed a significant difference in comparison with primary cultured mSTM neurons (155.4 ± 10.1 pA/pF): p < 3.0E − 13 and p < 6.0E − 15, respectively. (d) Comparison of capacitances of all three cell types. The mean values ± SEM (□) of undifferentiated mDPSCs (62.0 ± 10.2) and neuronally differentiated high nestin-expressing mDPSCs (30.7 ± 4.0), as well as undifferentiated mDPSCs and P0 mSTM neurons (20.9 ± 6.6), were considered as significantly different: p < 0.005 and p < 0.002, respectively. ● and ○ are maximal and minimal values, respectively.