Figure 4.

Only high nestin-expressing mDPSC clones display the ability to differentiate into oligodendrocyte-like cells. (a) Representative phase contrast images of high (clone 2) and low (clone 3) nestin-expressing clones following 15 days of oligodendrocyte-like differentiation. Clones with higher levels of nestin mRNA were found to adopt a more highly branched oligodendrocyte-like morphology compared to lower nestin-expressing clones. (b) Immunocytochemical staining identified the presence of myelin basic protein (MBP) and the oligodendrocyte transcription factors Olig1 and Olig2 in high, but not low, nestin-expressing mDPSC clones following 10 days of differentiation. β-actin staining was performed to demonstrate highly branched morphology. Scale bars = 100 μm.