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. 2016 Jun 7;7:11596. doi: 10.1038/ncomms11596

Figure 1. N. brasiliensis infection inhibits K/BxN SIA.

Figure 1

(a) Arthritis scores from WT mice infected or not with N. brasiliensis (Nb) (n=6–10 per group). (b,c) Hematoxylin and eosin (H&E) and tartrate-resistant acid phosphatase (TRAP) staining (b), and quantification of inflammation area, erosion area and number of osteoclasts per paw (N.Oc per paw) (c) in the hind paw of WT mice with or without Nb challenge at day 9 post serum transfer (n=6 per group). Scale bar, 500 μm. (d,e) Analyses of Acp5 (encoding TRAP), Ctsk (encoding cathepsin K), Tnfrsf11a (encoding RANK), Nfatc1 (encoding NFATc1) (d), Tnf, Il1b and Il6 (e) mRNA expression in synovial extracts and TNF and IL1β serum levels in WT mice with and without Nb challenge at day 9 post serum transfer (n=8–11 per group). (f) Frequency of CD4+IFN-γ+ (Th1), CD4+IL-4+ (Th2) and CD4+IL-17+ (Th17) cells in the spleen of WT mice with or without Nb challenge at day 9 post serum transfer (n=6–10 per group). (g–i) Frequency of IL-4+ (g), IL-13+ (h) and IL-5+ (i) lymphocytes in the spleen and in mesenteric lymph node (mLN) from WT mice with or without Nb challenge at the indicated time points (n=3–5 per group). Data are shown as mean±s.e.m. Pictures are representative of 3 independent experiments. Asterisks mark statistically significant difference (*P<0.05, **P<0.01 and ***P<0.001 determined by Student's t-test).