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. 2016 Jun 7;7:11758. doi: 10.1038/ncomms11758

Figure 4. CD44-sorted hGPCs colonized and replaced endogenous glia within the R6/2 × rag1−/− striatum.

Figure 4

Striatal engraftment of the R6/2 mice by CD44-sorted hGPCs was robust and dense. (a,b) Fetal derived cells expanded to colonize the striata and ventral forebrain of engrafted mice by 20 weeks. (c) Donor-derived cells in the striata of transplanted mice increased as a function of time (means±s.e.m.). (d–g) By 20 weeks after neonatal graft, the donor hGPCs (human nuclear antigen, red) integrated as astrocytes (d; GFAP, green) or persisted as GPCs (e,f; PDGFαR and olig2, green), but did not give rise to neurons; no overlap was ever seen of hNA and NeuN expression (g; NeuN, green). (h) Resident human glia did not manifest detectable nuclear Htt aggregates, as assessed by EM48 immunostaining; the staining patterns of host Htt and donor human nuclear antigen were always entirely non-overlapping (h). Scale bars, 1 mm (a,b); 25 μm (d–h).