Figure 1. Jmjd2a and Jmjd2b are individually dispensable for ESC self‐renewal and embryogenesis.

• A, B
  Schematic representation of the KO strategies used for the conditional deletion of Jmjd2a (A) and Jmjd2b (B). FRT: flippase recognition site, LoxP: Cre recombinase recognition site.
• C–E
  2a and 2b ESCs maintained in 2i medium were cultured in the absence (Ctrl) or presence of OHT and subsequently used for WB (C), growth curves and RT‐qPCR (D, E).
• F, G
  2a and 2b ESCs were adapted to grow in serum‐containing ESC medium, exposed to OHT as indicated and subsequently used for growth curves and RT‐qPCR analyses.
• H, I
  Jmjd2a(+/−); Jmjd2a(+/−) (H) or Jmjd2b(+/−); Jmjd2b(+/−) (I) mice were intercrossed and the number of pups alive at time of weaning are reported. P‐values were calculated using a chi‐square test.

Data information: Data in panels (C–G) are representative of results obtained with at least two independently derived ESC lines of each genotype. Graphs show mean ± standard deviation (SD) for three cultures counted in parallel (growth curves) or three technical replicates (RT‐qPCR).Source data are available online for this figure.