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. 2016 Jun 6;35(14):1550–1564. doi: 10.15252/embj.201593317

Figure 3. The combined functions of Jmjd2a and Jmjd2c are essential for early embryonic development.

Figure 3

  • A, B
    2ac ESCs expressing GFP were cultured in the absence or presence of OHT and subsequently used for morula injections. (A) Average volume of the GFP‐positive area in chimeric embryos at 24 and 72 h after morula injection. Number of embryos used for quantification: Ctrl 24 h n = 18; +OHT 24 h n = 15; Ctrl 72 h n = 5; +OHT 72 h n = 5. Data are presented as mean ± SD. (B) Representative images of chimeric embryos. The white bars indicate a length of 32 μm. Relative intensities of the GFP signal are indicated for each image.
  • C, D
    Jmjd2a(+/−);Jmjd2c(+/−) mice were intercrossed. The number of (C) pups alive at weaning or (D) embryos recovered at E6.5 is presented. P‐values were calculated using exact binomial test with the most conservative estimate of expected (−/−;−/−) embryos (6.25%, assuming no linkage between the Jmjd2a and Jmjd2c loci even though they are located on the same chromosome).